Commission Delegated Regulation (EU) 2021/1353 of 17 May 2021 supplementing Regulation (EU) 2017/625 of the European Parliament and of the Council with regard to the cases and conditions under which competent authorities may designate official laboratories which do not fulfil the conditions in relation to all the methods they use for official controls or other official activitiesText with EEA relevance.

Article 3 shall apply from 29 April 2022.

This Regulation shall be binding in its entirety and directly applicable in all Member States.

Done at Brussels, 17 May 2021.

For the Commission

The President

Ursula von der Leyen

Annex

ANNEX

CATEGORIES OF METHODS USED FOR ANALYSES, TESTS AND DIAGNOSES IN THE AREA OF PLANT HEALTH

1. Baiting, isolation and extraction methods, including:

Plating methods

Methods for extraction of target organism from matrix

1. Morphological and morphometric methods

1. Pathogenicity assessment methods

1. Biochemical methods, including:

Enzyme electrophoresis

Return-Polyacrylamide Gel Electrophoresis (R-PAGE)

1. Fingerprint methods, including:

Protein profiling

Fatty acid profiling

DNA profiling

MALDI-TOF mass spectrometry

1. Molecular methods, including:

Conventional Polymerase Chain Reaction (PCR)

Real-time Polymerase Chain Reaction (Real Time PCR)

Loop-mediated isothermal amplification (LAMP)

Recombinase polymerase amplification (RPA)

Next-generation sequencing (NGS)

DNA barcoding

1. Serological methods, including:

Immunofluorescence

Enzyme-linked immunosorbent assay (ELISA)

Immunosorbent electron microscopy